ACTA1 monoclonal antibody, clone 337CT30.10.1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CEM cell line lysates reacted with ACTA1 monoclonal antibody (Cat # MAB12316) at 1:100-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACTA1 monoclonal antibody (Cat # MAB12316) at 1:10-1:50 dilution.
Flow Cytometry
Flow cytometric analysis of A549 cells (right histogram) and a negative control cell (left histogram) reacted with ACTA1 monoclonal antibody (Cat # MAB12316) at 1:10-1:50 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human ACTA1.
Immunogen
Recombinant protein corresponding to human ACTA1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1:10-1:50)
Immunohistochemistry (1:10-1:50)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CEM cell line lysates reacted with ACTA1 monoclonal antibody (Cat # MAB12316) at 1:100-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACTA1 monoclonal antibody (Cat # MAB12316) at 1:10-1:50 dilution.Flow Cytometry
Flow cytometric analysis of A549 cells (right histogram) and a negative control cell (left histogram) reacted with ACTA1 monoclonal antibody (Cat # MAB12316) at 1:10-1:50 dilution. -
Gene Info — ACTA1
Entrez GeneID
58Gene Name
ACTA1
Gene Alias
ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
Gene Description
actin, alpha 1, skeletal muscle
Gene Ontology
HyperlinkGene Summary
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq
Other Designations
OTTHUMP00000036123|alpha skeletal muscle actin
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Interactome
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Disease
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