UCHL1 monoclonal antibody, clone 346CT2.2.1
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Specification
Product Description
Mouse monoclonal antibody raised against human UCHL1.
Immunogen
Recombinant protein corresponding to human UCHL1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Immunohistochemistry (1:25)
Immunofluorescence (1:25)
Western Blot (1:120-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: U266 cell line lysates Lane 2: mouse Neuro-2a whole cell lysates Lane 3: rat PC-12 whole cell lysates Lane 4: C6 cell line lysates Lane 5: mouse brain tissue lysates reacted with UCHL1 monoclonal antibody (Cat # MAB12315) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human kidney section reacted with UCHL1 monoclonal antibody (Cat # MAB12315) at 1:25 dilution.Immunofluorescence
Immunofluorescent staining of A549 cells reacted with UCHL1 monoclonal antibody (Cat # MAB12315) at 1:25 dilution. -
Gene Info — UCHL1
Entrez GeneID
7345Gene Name
UCHL1
Gene Alias
PARK5, PGP9.5, Uch-L1
Gene Description
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
Other Designations
ubiquitin C-terminal esterase L1|ubiquitin carboxyl-terminal esterase L1|ubiquitin thiolesterase L1
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Interactome
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Disease
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