ALDH1A1 monoclonal antibody, clone 152CT1.2.2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell line lysates Lane 2: K562 cell line lysates Lane 3: NCI-H460 cell line lysates reacted with ALDH1A1 monoclonal antibody (Cat # MAB12300) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human colon carcinoma reacted with ALDH1A1 monoclonal antibody (Cat # MAB12300) at 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of HepG2 cells reacted with ALDH1A1 monoclonal antibody (Cat # MAB12300) at 1:10-1:50 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human ALDH1A1.
Immunogen
Recombinant protein corresponding to human ALDH1A1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell line lysates Lane 2: K562 cell line lysates Lane 3: NCI-H460 cell line lysates reacted with ALDH1A1 monoclonal antibody (Cat # MAB12300) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human colon carcinoma reacted with ALDH1A1 monoclonal antibody (Cat # MAB12300) at 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of HepG2 cells reacted with ALDH1A1 monoclonal antibody (Cat # MAB12300) at 1:10-1:50 dilution. -
Gene Info — ALDH1A1
Entrez GeneID
216Gene Name
ALDH1A1
Gene Alias
ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1
Gene Description
aldehyde dehydrogenase 1 family, member A1
Gene Ontology
HyperlinkGene Summary
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq
Other Designations
ALDH class 1|acetaldehyde dehydrogenase 1|aldehyde dehydrogenase 1, soluble|aldehyde dehydrogenase 1A1|aldehyde dehydrogenase, liver cytosolic|retinal dehydrogenase 1|retinaldehyde dehydrogenase 1
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Interactome
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Pathway
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Disease
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