ALDH2 monoclonal antibody, clone 138CT22.3.8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NCI-H292 whole cell lysates Lane 2: A549 whole cell lysates Lane 3: HepG2 whole cell lysates reacted with ALDH2 monoclonal antibody (Cat # MAB12292) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH2 monoclonal antibody (Cat # MAB12292) at 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of HepG2 cell reacted with ALDH2 monoclonal antibody (Cat # MAB12292) at 1:10-1:50 dilution.
Flow Cytometry
Flow cytometric analysis of HepG2 cells (right histogram) and a negative control cell (left histogram) reacted with ALDH2 monoclonal antibody (Cat # MAB12292) at 1:10-1:50 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human ALDH2.
Immunogen
Recombinant protein corresponding to human ALDH2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1:10-1:50)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:10-1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NCI-H292 whole cell lysates Lane 2: A549 whole cell lysates Lane 3: HepG2 whole cell lysates reacted with ALDH2 monoclonal antibody (Cat # MAB12292) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH2 monoclonal antibody (Cat # MAB12292) at 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of HepG2 cell reacted with ALDH2 monoclonal antibody (Cat # MAB12292) at 1:10-1:50 dilution.Flow Cytometry
Flow cytometric analysis of HepG2 cells (right histogram) and a negative control cell (left histogram) reacted with ALDH2 monoclonal antibody (Cat # MAB12292) at 1:10-1:50 dilution. -
Gene Info — ALDH2
Entrez GeneID
217Gene Name
ALDH2
Gene Alias
ALDH-E2, ALDHI, ALDM, MGC1806
Gene Description
aldehyde dehydrogenase 2 family (mitochondrial)
Omim ID
100650Gene Ontology
HyperlinkGene Summary
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq
Other Designations
ALDH class 2|acetaldehyde dehydrogenase 2|liver mitochondrial ALDH|mitochondrial aldehyde dehydrogenase 2|nucleus-encoded mitochondrial aldehyde dehydrogenase 2
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Interactome
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Pathway
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Disease
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