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Last updated: 2017/6/25

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NCL monoclonal antibody, clone 130CT43.8.5

  • Catalog # : MAB12287
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human NCL.
  • Immunogen:
  • Recombinant protein corresponding to human NCL.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Immunofluorescence (1:10-1:50)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of 293 cell line lysates reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of formalin-fixed and paraffin-embedded human skeletal muscle reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of 293 cell reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:10-1:50 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4691
  • Gene Name:
  • NCL
  • Gene Alias:
  • C23,FLJ45706
  • Gene Description:
  • nucleolin
  • Gene Summary:
  • Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
  • Other Designations:
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