NCL monoclonal antibody, clone 130CT43.8.5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293 cell line lysates reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human skeletal muscle reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of 293 cell reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:10-1:50 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human NCL.
Immunogen
Recombinant protein corresponding to human NCL.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293 cell line lysates reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human skeletal muscle reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of 293 cell reacted with NCL monoclonal antibody (Cat # MAB12287) at 1:10-1:50 dilution. -
Gene Info — NCL
Entrez GeneID
4691Gene Name
NCL
Gene Alias
C23, FLJ45706
Gene Description
nucleolin
Omim ID
164035Gene Ontology
HyperlinkGene Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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