ATG12 monoclonal antibody, clone 43CT73.3.5.5.4
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of ATG12 recombinant protein reacted with ATG12 monoclonal antibody (Cat # MAB12281) at 1:200-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ATG12 monoclonal antibody (Cat # MAB12281) at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of HEK cells reacted with ATG12 monoclonal antibody (Cat # MAB12281) at 1:10-1:50 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human ATG12.
Immunogen
Recombinant protein corresponding to human ATG12.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG2b, kappa
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:10-1:50)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of ATG12 recombinant protein reacted with ATG12 monoclonal antibody (Cat # MAB12281) at 1:200-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ATG12 monoclonal antibody (Cat # MAB12281) at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of HEK cells reacted with ATG12 monoclonal antibody (Cat # MAB12281) at 1:10-1:50 dilution. -
Gene Info — ATG12
Entrez GeneID
9140Gene Name
ATG12
Gene Alias
APG12, APG12L, FBR93, HAPG12
Gene Description
ATG12 autophagy related 12 homolog (S. cerevisiae)
Omim ID
609608Gene Ontology
HyperlinkGene Summary
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM
Other Designations
APG12 autophagy 12-like|Apg12 (autophagy, yeast) homolog
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Interactome
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Pathway
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Disease
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