NANOG monoclonal antibody, clone 60CT77.1.1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K562 cell line lysates (35 ug/lane) reacted with NANOG monoclonal antibody (Cat # MAB12279) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human testis tissue reacted with NANOG monoclonal antibody (Cat # MAB12279) at 1:10-1:50 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human NANOG.
Immunogen
Recombinant protein corresponding to human NANOG.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, Kappa
Recommend Usage
Immunohistochemistry (1:10-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 cell line lysates (35 ug/lane) reacted with NANOG monoclonal antibody (Cat # MAB12279) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human testis tissue reacted with NANOG monoclonal antibody (Cat # MAB12279) at 1:10-1:50 dilution. -
Gene Info — NANOG
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Interactome
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Publication Reference
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Evaluation of immunohistochemical expression of stem cell markers (NANOG and CD133) in normal, hyperplastic, and malignant endometrium.
Methaq Al-Kaabi, Khalida Noel, Abdal-Jabbar Al-Rubai.
Journal of Medicine and Life 2022 Jan; 15(1):117.
Application：IHC-P, Human, Human endometrium.
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Evaluation of immunohistochemical expression of stem cell markers (NANOG and CD133) in normal, hyperplastic, and malignant endometrium.
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