GAPDH monoclonal antibody, clone 1A10A10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates reacted with GAPDH monoclonal antibody (Cat # MAB12275) at 1:2000-1:10000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human kidney section reacted with GAPDH monoclonal antibody (Cat # MAB12275) at 1:25 dilution.
Immunofluorescence
Immunofluorescent staining of Hela cell reacted with GAPDH monoclonal antibody (Cat # MAB12275) at 1:25 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human GAPDH.
Immunogen
Recombinant protein corresponding to human GAPDH.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:25)
Immunofluorescence (1:25)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates reacted with GAPDH monoclonal antibody (Cat # MAB12275) at 1:2000-1:10000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human kidney section reacted with GAPDH monoclonal antibody (Cat # MAB12275) at 1:25 dilution.Immunofluorescence
Immunofluorescent staining of Hela cell reacted with GAPDH monoclonal antibody (Cat # MAB12275) at 1:25 dilution. -
Gene Info — GAPDH
Entrez GeneID
2597Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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