Bpifa2 monoclonal antibody, clone 2B4F5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [Bpifa2 (AA: 16-169)-hIgGFc transfected HEK293 cells] with Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of Bpifa2 (AA: 16-169) recombinant protein (Expected MW is 18.5 kDa) with Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of prostate cancer tissues (A) and endometrial cencer tissues (B) with Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant mouse Bpifa2.
Immunogen
Recombinant protein corresponding to amino acids 16-169 of mouse Bpifa2.
Host
Mouse
Theoretical MW (kDa)
24.8
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [Bpifa2 (AA: 16-169)-hIgGFc transfected HEK293 cells] with Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of Bpifa2 (AA: 16-169) recombinant protein (Expected MW is 18.5 kDa) with Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of prostate cancer tissues (A) and endometrial cencer tissues (B) with Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with Bpifa2 monoclonal antibody, clone 2B4F5 (Cat# MAB12267) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — Psp
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