NT5E monoclonal antibody, clone 4G6E3
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human NT5E.
Immunogen
Recombinant protein corresponding to amino acids 30-250 of human TIE1.
Host
Mouse
Theoretical MW (kDa)
63.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A431 cell lysate with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [NT5E (AA: 30-250)-hIgGFc transfected HEK293 cells] with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human NT5E (AA: 30-250) recombinant protein (Expected MW is 26.6 kDa) with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human endometrial cancer tissues (A) and esophageal cencer tissues (B) with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:10000 dilution.Flow Cytometry
Flow Cytometry analysis of A431 cells with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — NT5E
Entrez GeneID
4907Gene Name
NT5E
Gene Alias
CD73, E5NT, NT, NT5, NTE, eN, eNT
Gene Description
5'-nucleotidase, ecto (CD73)
Omim ID
129190Gene Ontology
HyperlinkGene Summary
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM
Other Designations
5' nucleotidase (CD73)|5' nucleotidase, ecto|OTTHUMP00000016808|OTTHUMP00000040565|Purine 5-Prime-Nucleotidase|ecto-5'-nucleotidase
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