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Last updated: 2017/10/15

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NT5E monoclonal antibody, clone 4G6E3

  • Catalog # : MAB12266
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human NT5E.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 30-250 of human TIE1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 63.4
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of A431 cell lysate with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [NT5E (AA: 30-250)-hIgGFc transfected HEK293 cells] with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human NT5E (AA: 30-250) recombinant protein (Expected MW is 26.6 kDa) with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human endometrial cancer tissues (A) and esophageal cencer tissues (B) with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:200-1:1000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:10000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cytometry analysis of A431 cells with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:200-1:400 dilution (Green) and negative control (Red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4907
  • Gene Name:
  • NT5E
  • Gene Alias:
  • CD73,E5NT,NT,NT5,NTE,eN,eNT
  • Gene Description:
  • 5'-nucleotidase, ecto (CD73)
  • Gene Summary:
  • Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM
  • Other Designations:
  • 5' nucleotidase (CD73),5' nucleotidase, ecto,OTTHUMP00000016808,OTTHUMP00000040565,Purine 5-Prime-Nucleotidase,ecto-5'-nucleotidase
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