Western blot analysis of A431 cell lysate with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [NT5E (AA: 30-250)-hIgGFc transfected HEK293 cells] with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human NT5E (AA: 30-250) recombinant protein (Expected MW is 26.6 kDa) with NT5E monoclonal antibody, clone 4G6E3 (Cat# MAB12266) at 1:500-1:2000 dilution.
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 18.104.22.168) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM