CDKN2A monoclonal antibody, clone 1D7D2A1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell lysate; Lane 2: HepG2 cell lysate; Lane 3: Hek293 cell lysate with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CDKN2A (AA: 1-156)-hIgGFc transfected HEK293 cells] with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human CDKN2A (AA: 1-156) recombinant protein (Expected MW is 19 kDa) with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human endometrial cancer tissues (A) and esophageal cencer tissues (B) with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CDKN2A.
Immunogen
Recombinant protein corresponding to amino acids 1-156 of human CDKN2A.
Host
Mouse
Theoretical MW (kDa)
16.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell lysate; Lane 2: HepG2 cell lysate; Lane 3: Hek293 cell lysate with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CDKN2A (AA: 1-156)-hIgGFc transfected HEK293 cells] with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human CDKN2A (AA: 1-156) recombinant protein (Expected MW is 19 kDa) with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human endometrial cancer tissues (A) and esophageal cencer tissues (B) with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of Hela cells with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — CDKN2A
Entrez GeneID
1029Gene Name
CDKN2A
Gene Alias
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p16INK4a, p19
Gene Description
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Gene Ontology
HyperlinkGene Summary
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq
Other Designations
CDK4 inhibitor p16-INK4|OTTHUMP00000021147|OTTHUMP00000021148|cell cycle negative regulator beta|cyclin-dependent kinase inhibitor 2A|cyclin-dependent kinase inhibitor p16|multiple tumor suppressor 1
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Interactome
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Disease
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