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TIE1 monoclonal antibody, clone 8D12D2

  • Catalog # : MAB12262
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human TIE1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 385-607 of human TIE1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 125
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HepG2 cell lysate with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cells] with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human TIE1 (AA: 385-607) recombinant protein (Expected MW is 50.6 kDa) with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human ovarian cancer tissues (A) and kidney tissues (B) with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:200-1:1000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:10000 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7075
  • Gene Name:
  • TIE1
  • Gene Alias:
  • JTK14,TIE
  • Gene Description:
  • tyrosine kinase with immunoglobulin-like and EGF-like domains 1
  • Other Designations:
  • OTTHUMP00000008352,receptor tyrosine kinase,soluble TIE1 variant 1,soluble TIE1 variant 2,soluble TIE1 variant 3,soluble TIE1 variant 4,soluble TIE1 variant 5,tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
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