TIE1 monoclonal antibody, clone 8D12D2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cells] with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human TIE1 (AA: 385-607) recombinant protein (Expected MW is 50.6 kDa) with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human ovarian cancer tissues (A) and kidney tissues (B) with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:10000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human TIE1.
Immunogen
Recombinant protein corresponding to amino acids 385-607 of human TIE1.
Host
Mouse
Theoretical MW (kDa)
125
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cells] with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human TIE1 (AA: 385-607) recombinant protein (Expected MW is 50.6 kDa) with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human ovarian cancer tissues (A) and kidney tissues (B) with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:10000 dilution. -
Gene Info — TIE1
Entrez GeneID
7075Gene Name
TIE1
Gene Alias
JTK14, TIE
Gene Description
tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Omim ID
600222Gene Ontology
HyperlinkOther Designations
OTTHUMP00000008352|receptor tyrosine kinase|soluble TIE1 variant 1|soluble TIE1 variant 2|soluble TIE1 variant 3|soluble TIE1 variant 4|soluble TIE1 variant 5|tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
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Interactome
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Disease
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