CD68 monoclonal antibody, clone 3F7D3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: U937 cell lysate; Lane 2: Hela cell lysate; Lane 3: HepG2 cell lysate; Lane 4: Jurkat cell lysate with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CD68 (AA: 42-155)-hIgGFc transfected HEK293 cells] with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human CD68 (AA: 42-155) recombinant protein (Expected MW is 37.4 kDa) with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human endometrial cancer tissues (A) and bladder cancer tissues (B) with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:1000 dilution.
Immunofluorescence
Immunofluorescent staining of Hela cells with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CD68.
Immunogen
Recombinant protein corresponding to amino acids 42-155 of human CD68.
Host
Mouse
Theoretical MW (kDa)
37.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: U937 cell lysate; Lane 2: Hela cell lysate; Lane 3: HepG2 cell lysate; Lane 4: Jurkat cell lysate with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CD68 (AA: 42-155)-hIgGFc transfected HEK293 cells] with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human CD68 (AA: 42-155) recombinant protein (Expected MW is 37.4 kDa) with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human endometrial cancer tissues (A) and bladder cancer tissues (B) with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of Hela cells with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of Hela cells with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — CD68
Entrez GeneID
968Gene Name
CD68
Gene Alias
DKFZp686M18236, GP110, SCARD1
Gene Description
CD68 molecule
Omim ID
153634Gene Ontology
HyperlinkGene Summary
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq
Other Designations
CD68 antigen|OTTHUMP00000135285|macrophage antigen CD68|macrosialin|scavenger receptor class D, member 1
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Interactome
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Pathway
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