Mouse monoclonal antibody raised against partial recombinant human CD68.
Recombinant protein corresponding to amino acids 42-155 of human CD68.
Theoretical MW (kDa):
ELISA (1:10000) Flow Cytometry (1:200-1:400) Immunofluorescence (1:200-1:1000) Immunohistochemistry (1:200-1:1000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In PBS (0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: U937 cell lysate; Lane 2: Hela cell lysate; Lane 3: HepG2 cell lysate; Lane 4: Jurkat cell lysate with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CD68 (AA: 42-155)-hIgGFc transfected HEK293 cells] with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human CD68 (AA: 42-155) recombinant protein (Expected MW is 37.4 kDa) with CD68 monoclonal antibody, clone 3F7D3 (Cat# MAB12260) at 1:500-1:2000 dilution.
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq
CD68 antigen,OTTHUMP00000135285,macrophage antigen CD68,macrosialin,scavenger receptor class D, member 1