MSTN monoclonal antibody, clone 6E4B2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of LNcap cell lysate with MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human MSTN (AA:24-266) recombinant protein (Expected MW is 28.9 kDa) with MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cervical cancer tissues (A) and liver cancer tissues (B) with MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of LNcap cells with MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human MSTN.
Immunogen
Recombinant protein corresponding to amino acids 24-266 of human MSTN.
Host
Mouse
Theoretical MW (kDa)
42.8
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LNcap cell lysate with MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human MSTN (AA:24-266) recombinant protein (Expected MW is 28.9 kDa) with MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cervical cancer tissues (A) and liver cancer tissues (B) with MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of LNcap cells with MSTN monoclonal antibody, clone 6E4B2 (Cat# MAB12255) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — MSTN
Entrez GeneID
2660Gene Name
MSTN
Gene Alias
GDF8
Gene Description
myostatin
Omim ID
601788Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. [provided by RefSeq
Other Designations
growth differentiation factor 8
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Interactome
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Disease
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