DSG3 monoclonal antibody, clone 6G2C11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A431 cell lysate with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [DSG3 (AA: 55-159)-hIgGFc transfected HEK293 cells] with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human DSG3 (AA: 55-159) recombinant protein (Expected MW is 37.5 kDa) with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human esophageal cancer tissues (A) and esophageal tissues (B) with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of A431 cells with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human DSG3.
Immunogen
Recombinant protein corresponding to amino acids 55-159 of human DSG3.
Host
Mouse
Theoretical MW (kDa)
107.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A431 cell lysate with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [DSG3 (AA: 55-159)-hIgGFc transfected HEK293 cells] with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human DSG3 (AA: 55-159) recombinant protein (Expected MW is 37.5 kDa) with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human esophageal cancer tissues (A) and esophageal tissues (B) with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of A431 cells with DSG3 monoclonal antibody, clone 6G2C11 (Cat# MAB12254) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — DSG3
Entrez GeneID
1830Gene Name
DSG3
Gene Alias
CDHF6, DKFZp686P23184, PVA
Gene Description
desmoglein 3 (pemphigus vulgaris antigen)
Omim ID
169615Gene Ontology
HyperlinkGene Summary
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq
Other Designations
130-kD pemphigus vulgaris antigen|desmoglein 3|pemphigus vulgaris antigen
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Interactome
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Disease
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