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PLA2G12A monoclonal antibody, clone 7C7C9

  • Catalog # : MAB12253
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human PLA2G12A.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 21-189 of human PLA2G12A.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 21
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 cells] with PLA2G12A monoclonal antibody, clone 7C7C9 (Cat# MAB12253) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human PLA2G12A (AA: 21-189) recombinant protein (Expected MW is 44.5 kDa) with PLA2G12A monoclonal antibody, clone 7C7C9 (Cat# MAB12253) at 1:500-1:2000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of PLA2G12A monoclonal antibody, clone 7C7C9 (Cat# MAB12253) at 1:10000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cytometry analysis of Hela cells with PLA2G12A monoclonal antibody, clone 7C7C9 (Cat# MAB12253) at 1:200-1:400 dilution (Green) and negative control (Red).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Gene Name:
  • PLA2G12A
  • Gene Alias:
  • GXII,PLA2G12,ROSSY
  • Gene Description:
  • phospholipase A2, group XIIA
  • Gene Summary:
  • Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM
  • Other Designations:
  • group XII secreted phospholipase A2,group XIIA secreted phospholipase A2
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