HOXB4 monoclonal antibody, clone 3A2F2
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant human HOXB4.
Immunogen
Recombinant protein corresponding to amino acids 16-251 of human HOXB4.
Host
Mouse
Theoretical MW (kDa)
27.6
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [HOXB4 (AA: 16-251)-hIgGFc transfected HEK293 cells] with HOXB4 monoclonal antibody, clone 3A2F2 (Cat# MAB12252) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human HOXB4 (AA: 16-251) recombinant protein (Expected MW is 51.9 kDa) with HOXB4 monoclonal antibody, clone 3A2F2 (Cat# MAB12252) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum cancer tissues (A) and colon cancer tissues (B) with HOXB4 monoclonal antibody, clone 3A2F2 (Cat# MAB12252) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HOXB4 monoclonal antibody, clone 3A2F2 (Cat# MAB12252) at 1:10000 dilution. -
Gene Info — HOXB4
Entrez GeneID
3214Gene Name
HOXB4
Gene Alias
HOX-2.6, HOX2, HOX2F
Gene Description
homeobox B4
Omim ID
142965Gene Ontology
HyperlinkGene Summary
This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Intracellular or ectopic expression of this protein expands hematopoietic stem and progenitor cells in vivo and in vitro, making it a potential candidate for therapeutic stem cell expansion. [provided by RefSeq
Other Designations
homeo box 2F|homeo box B4
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