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Last updated: 2016/11/27

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EPCAM monoclonal antibody, clone 7B5C10

  • Catalog # : MAB12251
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human EPCAM.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 24-265 of human EPCAM.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 35
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: A431 cell lysate; Lane 2: MCF-7 cell lysate with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [EPCAM (AA: 24-265)-hIgGFc transfected HEK293 cells] with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human EPCAM (AA: 24-265) recombinant protein (Expected MW is 53.4 kDa) with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human cervical cancer tissues (A) and esophageal cancer tissues (B) with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:200-1:1000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:10000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cytometry analysis of A431 cells with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:200-1:400 dilution (Green) and negative control (Red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4072
  • Gene Name:
  • EPCAM
  • Gene Alias:
  • 17-1A,323/A3,CD326,CO-17A,CO17-1A,EGP,EGP-2,EGP34,EGP40,ESA,Ep-CAM,GA733-2,HEA125,KS1/4,KSA,M4S1,MH99,MIC18,MK-1,MOC31,TACST-1,TACSTD1,TROP1,hEGP-2
  • Gene Description:
  • epithelial cell adhesion molecule
  • Gene Summary:
  • This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
  • Other Designations:
  • adenocarcinoma-associated antigen,carcinoma-associated antigen GA733-2,human epithelial glycoprotein-2,membrane component, chromosome 4, surface marker (35kD glycoprotein),tumor-associated calcium signal transducer 1
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