EPCAM monoclonal antibody, clone 7B5C10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell lysate; Lane 2: MCF-7 cell lysate with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [EPCAM (AA: 24-265)-hIgGFc transfected HEK293 cells] with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human EPCAM (AA: 24-265) recombinant protein (Expected MW is 53.4 kDa) with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cervical cancer tissues (A) and esophageal cancer tissues (B) with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of A431 cells with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human EPCAM.
Immunogen
Recombinant protein corresponding to amino acids 24-265 of human EPCAM.
Host
Mouse
Theoretical MW (kDa)
35
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell lysate; Lane 2: MCF-7 cell lysate with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [EPCAM (AA: 24-265)-hIgGFc transfected HEK293 cells] with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human EPCAM (AA: 24-265) recombinant protein (Expected MW is 53.4 kDa) with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cervical cancer tissues (A) and esophageal cancer tissues (B) with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of A431 cells with EPCAM monoclonal antibody, clone 7B5C10 (Cat# MAB12251) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — EPCAM
Entrez GeneID
4072Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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