PLA2G12A monoclonal antibody, clone 3H2C11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 cells] with PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human PLA2G12A (AA: 21-189) recombinant protein (Expected MW is 44.5 kDa) with PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver cancer tissues with PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells with PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PLA2G12A.
Immunogen
Recombinant protein corresponding to amino acids 21-189 of human PLA2G12A.
Host
Mouse
Theoretical MW (kDa)
21
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 cells] with PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human PLA2G12A (AA: 21-189) recombinant protein (Expected MW is 44.5 kDa) with PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver cancer tissues with PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of Hela cells with PLA2G12A monoclonal antibody, clone 3H2C11 (Cat# MAB12250) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — PLA2G12A
Entrez GeneID
81579Gene Name
PLA2G12A
Gene Alias
GXII, PLA2G12, ROSSY
Gene Description
phospholipase A2, group XIIA
Omim ID
611652Gene Ontology
HyperlinkGene Summary
Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM
Other Designations
group XII secreted phospholipase A2|group XIIA secreted phospholipase A2
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Interactome
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Pathway
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Disease
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