SMCP monoclonal antibody, clone 5C10D8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [SMCP (AA: 1-116)-hIgGFc transfected HEK293 cells] with SMCP monoclonal antibody, clone 5C10D8 (Cat# MAB12248) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human SMCP (AA: 1-116) recombinant protein (Expected MW is 38.3 kDa) with SMCP monoclonal antibody, clone 5C10D8 (Cat# MAB12248) at 1:500-1:2000 dilution.
Immunofluorescence
Immunofluorescent staining of Hela cells with SMCP monoclonal antibody, clone 5C10D8 (Cat# MAB12248) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SMCP monoclonal antibody, clone 5C10D8 (Cat# MAB12248) at 1:10000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human SMCP.
Immunogen
Recombinant protein corresponding to full length human SMCP.
Host
Mouse
Theoretical MW (kDa)
12.8
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [SMCP (AA: 1-116)-hIgGFc transfected HEK293 cells] with SMCP monoclonal antibody, clone 5C10D8 (Cat# MAB12248) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human SMCP (AA: 1-116) recombinant protein (Expected MW is 38.3 kDa) with SMCP monoclonal antibody, clone 5C10D8 (Cat# MAB12248) at 1:500-1:2000 dilution.Immunofluorescence
Immunofluorescent staining of Hela cells with SMCP monoclonal antibody, clone 5C10D8 (Cat# MAB12248) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SMCP monoclonal antibody, clone 5C10D8 (Cat# MAB12248) at 1:10000 dilution. -
Gene Info — SMCP
Entrez GeneID
4184Gene Name
SMCP
Gene Alias
HSMCSGEN1, MCS, MCSP, MGC26305, MGC26519
Gene Description
sperm mitochondria-associated cysteine-rich protein
Omim ID
601148Gene Ontology
HyperlinkGene Summary
Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. The protein encoded by this gene localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath. [provided by RefSeq
Other Designations
OTTHUMP00000014403|mitochondrial capsule selenoprotein
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