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Last updated: 2017/9/17

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CTNNBL1 monoclonal antibody, clone 1E4F5

  • Catalog # : MAB12247
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human CTNNBL1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 390-557 of human CTNNBL1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 65.2
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunofluorescence (1:200-1:1000)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Hela cell lysate; Lane 2: Jurkat cell lysate; Lane 3: HEK293 cell lysate; Lane 4: A431 cell lysate; Lane 5: HepG2 cell lysate; Lane 6: RAJI cell lysate with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CTNNBL1 (AA: 390-557)-hIgGFc transfected HEK293 cells] with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human CTNNBL1 (AA: 390-557) recombinant protein (Expected MW is 45.8 kDa) with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human colon cancer tissues (A) and esophageal cancer tissues (B) with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:1000 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of MCF-7 cells with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:1000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:10000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cytometry analysis of Hela cells with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:400 dilution (Green) and negative control (Red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • CTNNBL1
  • Gene Alias:
  • C20orf33,FLJ21108,NAP,NYD-SP19,P14L,PP8304,dJ633O20.1
  • Gene Description:
  • catenin, beta like 1
  • Gene Summary:
  • The protein encoded by this gene contains an acidic domain, a putative bipartite nuclear localization signal, a nuclear export signal, a leucine-isoleucine zipper, and phosphorylation motifs. In addition, the encoded protein contains Armadillo/beta-catenin-like repeats, which have been implicated in protein-protein interactions. Although the function of this protein has not been determined, the C-terminal portion of the protein has been shown to possess apoptosis-inducing activity. [provided by RefSeq
  • Other Designations:
  • beta catenin-like 1,nuclear associated protein
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