MEN1 monoclonal antibody, clone 7D3E10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [MEN1 (AA: 392-554)-hIgGFc transfected HEK293 cells] with MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human MEN1 (AA: 392-554) recombinant protein (Expected MW is 43.3 kDa) with MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum cancer tissues with MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells with MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human MEN1.
Immunogen
Recombinant protein corresponding to amino acids 392-554 of human MEN1.
Host
Mouse
Theoretical MW (kDa)
68
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [MEN1 (AA: 392-554)-hIgGFc transfected HEK293 cells] with MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human MEN1 (AA: 392-554) recombinant protein (Expected MW is 43.3 kDa) with MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum cancer tissues with MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of Hela cells with MEN1 monoclonal antibody, clone 7D3E10 (Cat# MAB12246) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — MEN1
Entrez GeneID
4221Gene Name
MEN1
Gene Alias
MEAI, SCG2
Gene Description
multiple endocrine neoplasia I
Gene Ontology
HyperlinkGene Summary
This gene encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000042868|OTTHUMP00000070176|OTTHUMP00000070180|OTTHUMP00000070184|endocrine adenomatosis, multiple|menin
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Interactome
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Disease
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