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RAB27A monoclonal antibody, clone 7D7C9

  • Catalog # : MAB12243
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human RAB27A.
  • Immunogen:
  • Recombinant protein corresponding to full length human RAB27A.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 24.9
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • ELISA (1:10000)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [RAB27A (AA: 1-221)-hIgGFc transfected HEK293 cells] with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human RAB27A (AA: 1-221) recombinant protein (Expected MW is 50.8 kDa) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human prostate cancer tissues (A) and liver cancer tissues (B) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:200-1:1000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:10000 dilution.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5873
  • Gene Name:
  • RAB27A
  • Gene Alias:
  • GS2,HsT18676,MGC117246,RAB27,RAM
  • Gene Description:
  • RAB27A, member RAS oncogene family
  • Gene Summary:
  • The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
  • Other Designations:
  • GTP-binding protein Ram,Ras-related protein Rab-27A
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