RAB27A monoclonal antibody, clone 7D7C9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [RAB27A (AA: 1-221)-hIgGFc transfected HEK293 cells] with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human RAB27A (AA: 1-221) recombinant protein (Expected MW is 50.8 kDa) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human prostate cancer tissues (A) and liver cancer tissues (B) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:10000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human RAB27A.
Immunogen
Recombinant protein corresponding to full length human RAB27A.
Host
Mouse
Theoretical MW (kDa)
24.9
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [RAB27A (AA: 1-221)-hIgGFc transfected HEK293 cells] with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human RAB27A (AA: 1-221) recombinant protein (Expected MW is 50.8 kDa) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human prostate cancer tissues (A) and liver cancer tissues (B) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:10000 dilution. -
Gene Info — RAB27A
Entrez GeneID
5873Gene Name
RAB27A
Gene Alias
GS2, HsT18676, MGC117246, RAB27, RAM
Gene Description
RAB27A, member RAS oncogene family
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
Other Designations
GTP-binding protein Ram|Ras-related protein Rab-27A
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Interactome
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Disease
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