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CCNE1 monoclonal antibody, clone 5F8C5

  • Catalog # : MAB12242
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human CCNE1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 307-410 of human CCNE1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 47
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Hela cell lysate; Lane 2: K562 cell lysate; Lane 3: NIH/3T3 cell lysate; Lane 4: C6 cell lysate; Lane 5: MCF-7 cell lysate; Lane 6: Jurkat cell lysate; Lane 7: A431 cell lysate with CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:500-1:2000 dilution.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CCNE1 (AA: 307-410)-hIgGFc transfected HEK293 cells] with CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human CCNE1 (AA:307-410) recombinant protein (Expected MW is 37.5 kDa) with CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:500-1:2000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:10000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cytometry analysis of K562 cells with CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:200-1:400 dilution (Green) and negative control (Red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 898
  • Gene Name:
  • CCNE1
  • Gene Alias:
  • CCNE
  • Gene Description:
  • cyclin E1
  • Gene Summary:
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq
  • Other Designations:
  • cyclin Es,cyclin Et
  • Interactome
  • Interactome
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