HPRT1 monoclonal antibody, clone 5F11A7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell lysate; Lane 2: A431 cell lysate; Lane 3: A549 cell lysate with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [HPRT1 (AA: 1-218)-hIgGFc transfected HEK293 cells] with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human HPRT1 (AA:1-218) recombinant protein (Expected MW is 50.5 kDa) with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney tissues with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human HPRT1.
Immunogen
Recombinant protein corresponding to full length human HPRT1.
Host
Mouse
Theoretical MW (kDa)
24.6
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell lysate; Lane 2: A431 cell lysate; Lane 3: A549 cell lysate with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [HPRT1 (AA: 1-218)-hIgGFc transfected HEK293 cells] with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human HPRT1 (AA:1-218) recombinant protein (Expected MW is 50.5 kDa) with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney tissues with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of Hela cells with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — HPRT1
Entrez GeneID
3251Gene Name
HPRT1
Gene Alias
HGPRT, HPRT
Gene Description
hypoxanthine phosphoribosyltransferase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout
Other Designations
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Interactome
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Pathway
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Disease
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