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Last updated: 2017/9/17

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HPRT1 monoclonal antibody, clone 5F11A7

  • Catalog # : MAB12241
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human HPRT1.
  • Immunogen:
  • Recombinant protein corresponding to full length human HPRT1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 24.6
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Hela cell lysate; Lane 2: A431 cell lysate; Lane 3: A549 cell lysate with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [HPRT1 (AA: 1-218)-hIgGFc transfected HEK293 cells] with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human HPRT1 (AA:1-218) recombinant protein (Expected MW is 50.5 kDa) with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human kidney tissues with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:200-1:1000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:10000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cytometry analysis of Hela cells with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:200-1:400 dilution (Green) and negative control (Red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3251
  • Gene Name:
  • HPRT1
  • Gene Alias:
  • HGPRT,HPRT
  • Gene Description:
  • hypoxanthine phosphoribosyltransferase 1
  • Gene Summary:
  • The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout
  • Other Designations:
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