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Last updated: 2016/12/11

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EIF2AK2 monoclonal antibody, clone 6H3A10

  • Catalog # : MAB12238
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human EIF2AK2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 329-551 of human EIF2AK2.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 62
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: A431 cell lysate; Lane 2: THP-1 cell lysate; Lane 3: MCF-7 cell lysate; Lane 4: PC-12 cell lysate with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [EIF2AK2 (AA: 329-551)-hIgGFc transfected HEK293 cells] with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human EIF2AK2 (AA: 329-551) recombinant protein (Expected MW is 51.7 kDa) with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human cervical cancer tissues (A) and rectum cancer tissues (B) with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:200-1:1000 dilution.
  • ELISA
  • ELISA
  • ELISA analysis of EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:10000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cytometry analysis of A431 cells with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:200-1:400 dilution (Green) and negative control (Red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5610
  • Gene Name:
  • EIF2AK2
  • Gene Alias:
  • EIF2AK1,MGC126524,PKR,PRKR
  • Gene Description:
  • eukaryotic translation initiation factor 2-alpha kinase 2
  • Other Designations:
  • OTTHUMP00000126954,double stranded RNA activated protein kinase,interferon-inducible elF2alpha kinase,protein kinase, interferon-inducible double stranded RNA dependent
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