EIF2AK2 monoclonal antibody, clone 6H3A10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell lysate; Lane 2: THP-1 cell lysate; Lane 3: MCF-7 cell lysate; Lane 4: PC-12 cell lysate with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [EIF2AK2 (AA: 329-551)-hIgGFc transfected HEK293 cells] with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human EIF2AK2 (AA: 329-551) recombinant protein (Expected MW is 51.7 kDa) with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cervical cancer tissues (A) and rectum cancer tissues (B) with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of A431 cells with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human EIF2AK2.
Immunogen
Recombinant protein corresponding to amino acids 329-551 of human EIF2AK2.
Host
Mouse
Theoretical MW (kDa)
62
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell lysate; Lane 2: THP-1 cell lysate; Lane 3: MCF-7 cell lysate; Lane 4: PC-12 cell lysate with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [EIF2AK2 (AA: 329-551)-hIgGFc transfected HEK293 cells] with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human EIF2AK2 (AA: 329-551) recombinant protein (Expected MW is 51.7 kDa) with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cervical cancer tissues (A) and rectum cancer tissues (B) with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of A431 cells with EIF2AK2 monoclonal antibody, clone 6H3A10 (Cat# MAB12238) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — EIF2AK2
Entrez GeneID
5610Gene Name
EIF2AK2
Gene Alias
EIF2AK1, MGC126524, PKR, PRKR
Gene Description
eukaryotic translation initiation factor 2-alpha kinase 2
Omim ID
176871Gene Ontology
HyperlinkGene Summary
interferon-inducible double stranded RNA dependent
Other Designations
OTTHUMP00000126954|double stranded RNA activated protein kinase|interferon-inducible elF2alpha kinase|protein kinase, interferon-inducible double stranded RNA dependent
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Interactome
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Disease
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