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Hemoglobin Alpha Chain monoclonal antibody, clone SP212

  • Catalog # : MAB12178
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of hemoglobin alpha chain.
  • Immunogen:
  • A synthetic peptide corresponding to human Hemoglobin Alpha Chain .
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 14
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
    Western Blot (Tissue lysate) (1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS, pH 7.6 (1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of human heart tissue lysate with Hemoglobin Alpha Chain monoclonal antibody, clone SP212 (Cat # MAB12178).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-Fixed Paraffin-Embedded Sections) staining of human placenta with Hemoglobin Alpha Chain monoclonal antibody, clone SP212 (Cat # MAB12178).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K562 cells with Hemoglobin Alpha Chain monoclonal antibody, clone SP212 (Cat # MAB12178).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3039
  • Gene Name:
  • HBA1
  • Gene Alias:
  • HBH,HBA-T3
  • Gene Description:
  • hemoglobin, alpha 1
  • Gene Summary:
  • The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq
  • Other Designations:
  • alpha 1 globin,alpha one globin,alpha-1 globin,alpha-1-globin,hemoglobin alpha 1 globin chain,hemoglobin alpha-1 chain
  • Gene Information
  • Entrez GeneID:
  • 3040
  • Gene Name:
  • HBA2
  • Gene Alias:
  • -
  • Gene Description:
  • hemoglobin, alpha 2
  • Gene Summary:
  • The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq
  • Other Designations:
  • alpha 2 globin,alpha globin,alpha-2 globin
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