Product Browser

Last updated: 2020/3/27
  • Related Product Showcase
  • By Application

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

CD44 variant isoform 9 (v9) monoclonal antibody, clone RV3 

  • Catalog # : MAB12149
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rat monoclonal antibody raised against human CD44 variant isoform 9 (v9).

    Features of clone RV3:
    1. Clone RV3 recognizes CD44 splice isoform 9 (CD44v9), which is upregulated specifically and frequently in various cancer cells.
    2. Clone RV3, the only CD44v9 antibody applicable for CSC enrichment in the world, can be used to concentrate cancer stem cells (CSCs) due to high expression of CD44v9 usually found in CSCs.
    3. Clone RV3 has been commonly used to detect existence of CD44v9 in cancer cells and CSCs in most influential journals of cancer research.
  • Immunogen:
  • Human CD44 variant isoform 8-10 transfected cell.
  • Host:
  • Rat
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Flow cytomerty (1-10 ug/mL)
    Cancer stem cell enrichment
    Immunohistochemistry (0.2 ug/mL)
    Immunofluorescence (3 ug/mL)
    Western blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS
  • Storage Instruction:
  • Store at 2-8°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Cancer Stem Cell Enrichment
  • Cancer Stem Cells (CSCs) have been identified as a subset of distinct population in many tumors and can drive cancer relapse and metastasis with their abilities to self-renew and differentiate into various types of cancer cells. Development of therapies specifically and effectively targeting CSCs thus is necessary to improve the survival and quality of life for cancer patients, especially for patients with metastatic diseases.
    Clone RV3 can be used for enrichment of CSCs with a cell sorter, and further be applied for studying mechanisms by which CSCs cause cancer relapse and metastasis to develop new drugs against CSCs with “in vitro sphere forming assay”, “in vivo lung metastasis assay”, etc.

    In vitro evaluation of CSCs by “sphere formation assay"
    In vitro sphere formation assays are colony formation assays used to evaluate the clonogenic potential of CSCs, and can be used to evaluate the potential effects of drugs. The figure above shows the sphere formation ability of CD44v9-High and CD44v9-Low cell populations sorted from a human prostate cancer cell line (PC-3) by using anti-CD44v9 monoclonal antibody, clone RV3 (Cat # MAB12149). The increased number of spheres observed in CD44v9-High population (CSC enriched) allows further accurate assessment for the effects of inhibitory drugs and other treatments on CSC sphere formation.

    In vivo evaluation of CSCs by "lung metastasis assay"
    Lung metastasis assay, injecting tumorigenic cells into mice intravenously, provides an opportunity to assess the inhibitory effects of compounds or other treatments on the ability of CSC to colonize. Injection of sorted CD44v9-High cells significantly increased the efficiency of metastasis formation and would be applied to evaluate the effectiveness of new anti-CSC therapeutic strategies.

  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: no treated, Lane 2: non-targeting siRNA treated sample, Lane 3: CD44 siRNA treated sample #1, Lane 4: CD44 siRNA treated sample #2 with CD44 (v9) monoclonal antibody, clone RV3 (Cat # MAB12149) at 1 ug/mL concentration.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of breast invasive ductal carcinoma with with CD44 (v9) monoclonal antibody, clone RV3 (Cat # MAB12149) at 0.2 ug/mL concentration.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of human mammary adenocarcinoma cells with CD44 (v9) monoclonal antibody, clone RV3 (Cat # MAB12149) at 3 ug/mL concentration. (left)
    Merged image with DAPI staining (right).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of human cancer cell line with CD44 (v9) monoclonal antibody, clone RV3 (Cat # MAB12149) at 3 ug/mL concentration and PE-labeled anti Rat IgG antibody.
  • Flow Cytometry
  • Flow Cytometry enlargeenlarge this image
  • CSC enrichment from human cancer cell lines using anti-CD44v9 monoclonal antibody, clone RV3 (Cat # MAB12149) at 3 ug/mL.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 960
  • Gene Name:
  • CD44
  • Gene Alias:
  • Gene Description:
  • CD44 molecule (Indian blood group)
  • Gene Summary:
  • The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
  • Other Designations:
  • CD44 antigen,CD44 antigen (homing function and Indian blood group system),CDW44 antigen,GP90 lymphocyte homing/adhesion receptor,Hermes antigen,antigen gp90 homing receptor,cell adhesion molecule,cell surface glycoprotein CD44,chondroitin sulfate proteogl
  • RSS
  • YouTube
  • Linkedin
  • Facebook