EPCAM monoclonal antibody, clone SPM134
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Specification
Product Description
Mouse monoclonal antibody raised against EPCAM.
Immunogen
Human lung carcinoma cells.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells)
Immunohistochemistry (0.5-1 ug/mL)
Western blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon carcinoma with EPCAM monoclonal antibody, clone SPM134 (Cat # MAB12106).Flow Cytometry
Surface staining of human HT29 with EPCAM monoclonal antibody, clone SPM134 (Cat # MAB12106) at 1 ug/106 cells. -
Gene Info — EPCAM
Entrez GeneID
4072Protein Accession#
P16422Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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Publication Reference
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Interpolation method for accurate affinity ranking of arrayed ligand-analyte interactions.
Schasfoort RBM, Andree KC, van der Velde N, van der Kooi A, Stojanovic I, Terstappen LWMM.
Analytical Biochemistry 2016 May; 500:21.
Application:SPR, Human, Purified protein.
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Interpolation method for accurate affinity ranking of arrayed ligand-analyte interactions.
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