CD5 monoclonal antibody, clone SPM546
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Specification
Product Description
Mouse monoclonal antibody raised against CD5.
Immunogen
Recombinant protein corresponding to human CD5.
Host
Mouse
Theoretical MW (kDa)
67
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) ((1-2 ug/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes))
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with CD5 monoclonal antibody, clone SPM546 (Cat # MAB12027).Immunofluorescence
Flow Cytometry
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Gene Info — CD5
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Interactome
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Pathway
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Disease
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