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Last updated: 2018/1/14
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PRDM2 monoclonal antibody, clone 33AT1045

  • Catalog # : MAB1151
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant PRDM2.
  • Immunogen:
  • Recombinant GST fusion protein corresponding to amino acids 1-347 of human PRDM2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PRDM2 monoclonal antibody, clone 33AT1045 (Cat # MAB1151) in lysate from transformed cells. Lane A : mock plasmid, Lane B : PRDM2 [1-347]-encoding plasmid. PRDM2 [1-347] (arrow) was detected using purified monoclonal antibody. Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.
  • Immunoprecipitation
  • Immunoprecipitation
  • A : Soluble chromatin was prepared from MCF-7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with PRDM2 monoclonal antibody, clone 33AT1045 (Cat # MAB1151). DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.
    B : Time course analysis of PRDM2 binding to the pS2 gene promoter. MCF-7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7799
  • GeneBank Accession#:
  • NM_012231;NM_01586
  • Gene Name:
  • PRDM2
  • Gene Alias:
  • Gene Description:
  • PR domain containing 2, with ZNF domain
  • Gene Summary:
  • This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • GATA-3 binding protein G3B,MTE-binding protein,OTTHUMP00000009642,OTTHUMP00000009687,retinoblastoma protein-binding zinc finger protein,retinoblastoma protein-interacting zinc finger protein,zinc-finger DNA-binding protein
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