H3F3A/H3F3B (phospho S10) monoclonal antibody, clone 44AT1232
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More Files
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Western Blot (Cell lysate)
Western analysis of extracts from HL-60 cells treated with 100 nM of calyculin using H3F3A/H3F3B (phospho S10) monoclonal antibody, clone 44AT1232 (Cat # MAB1148).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of H3F3A/H3F3B.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to amino acids 7-18 residues surrounding S10 of human H3F3A/H3F3B.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western analysis of extracts from HL-60 cells treated with 100 nM of calyculin using H3F3A/H3F3B (phospho S10) monoclonal antibody, clone 44AT1232 (Cat # MAB1148). -
Gene Info — H3F3A
Entrez GeneID
3020Protein Accession#
NP_002098;NP_005315;P84243Gene Name
H3F3A
Gene Alias
H3.3A, H3F3, MGC87782, MGC87783
Gene Description
H3 histone, family 3A
Omim ID
601128Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq
Other Designations
OTTHUMP00000035618|OTTHUMP00000035619|OTTHUMP00000035621
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Gene Info — H3F3B
Entrez GeneID
3021Protein Accession#
NP_002098;NP_005315;P84243Gene Name
H3F3B
Gene Alias
H3.3B, H3F3A
Gene Description
H3 histone, family 3B (H3.3B)
Omim ID
601058Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq
Other Designations
H3 histone, family 3A|H3 histone, family 3B
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Interactome
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Pathway
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Disease
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Publication Reference
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Distinct factors control histone variant H3.3 localization at specific genomic regions.
Goldberg AD, Banaszynski LA, Noh KM, Lewis PW, Elsaesser SJ, Stadler S, Dewell S, Law M, Guo X, Li X, Wen D, Chapgier A, DeKelver RC, Miller JC, Lee YL, Boydston EA, Holmes MC, Gregory PD, Greally JM, Rafii S, Yang C, Scambler PJ, Garrick D, Gibbons RJ, Higgs DR, Cristea IM, Urnov FD, Zheng D, Allis CD.
Cell 2010 Mar; 140(5):678.
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New functions for an old variant: no substitute for histone H3.3.
Elsaesser SJ, Goldberg AD, Allis CD.
Current Opinion in Genetics & Development 2010 Feb; 20(2):110.
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ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells.
Wong LH, McGhie JD, Sim M, Anderson MA, Ahn S, Hannan RD, George AJ, Morgan KA, Mann JR, Choo KH.
Genome Research 2010 Jan; 20(3):351.
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Distinct factors control histone variant H3.3 localization at specific genomic regions.
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