Products

Product Description

Mouse monoclonal antibody raised against DMPO.

Immunogen

5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated with ovalbumin.

Host

Mouse

Specificity

Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

Form

Liquid

Conjugation

APC

Purification

Protein G purification

Isotype

IgG1

Recommend Usage

ELISA (1:100)
Immunocytochemistry/Immunofluorescence (1:100)
Immunoprecipitation (25 ug)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Storage Buffer

In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated Antibody

Immunofluorescence

Immunofluorescent staining of mouse macrophage cells with DMPO monoclonal antibody, clone N1664A (Cat # MAB11426) at 10 ug/mL.

Western Blot (Recombinant protein)

Western blot analysis of Lane 1: 10 uM Hb (Hemoglobin), Lane 2: 10 uM Hb + 100 uM HOCL, Lane 3: 10 uM Hb + 500 uM HOCL, Lane 4: 10 uM Hb + 1000 uM HOCL, Lane 5-8: as above with 20 mM DMPO, Lane 9-12: as above with 100 mM DMPO with DMPO monoclonal antibody, clone N1664A (Cat # MAB11426).