DMPO monoclonal antibody, clone N1664A
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: 10 uM Hb (Hemoglobin), Lane 2: 10 uM Hb + 100 uM HOCL, Lane 3: 10 uM Hb + 500 uM HOCL, Lane 4: 10 uM Hb + 1000 uM HOCL, Lane 5-8: as above with 20 mM DMPO, Lane 9-12: as above with 100 mM DMPO with DMPO monoclonal antibody, clone N1664A (Cat # MAB11426).
Immunofluorescence
Immunofluorescent staining of mouse macrophage cells with DMPO monoclonal antibody, clone N1664A (Cat # MAB11426) at 10 ug/mL.
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Specification
Product Description
Mouse monoclonal antibody raised against DMPO.
Immunogen
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated with ovalbumin.
Host
Mouse
Specificity
Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA (1:100)
Immunocytochemistry/Immunofluorescence (1:100)
Immunoprecipitation (25 ug)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: 10 uM Hb (Hemoglobin), Lane 2: 10 uM Hb + 100 uM HOCL, Lane 3: 10 uM Hb + 500 uM HOCL, Lane 4: 10 uM Hb + 1000 uM HOCL, Lane 5-8: as above with 20 mM DMPO, Lane 9-12: as above with 100 mM DMPO with DMPO monoclonal antibody, clone N1664A (Cat # MAB11426).Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of mouse macrophage cells with DMPO monoclonal antibody, clone N1664A (Cat # MAB11426) at 10 ug/mL.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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