Rescue of the JNK pathway by expression of wild-type GAB1 in GAB1-/- cells. GAB1-/- cells were transiently transfected with 1, 2, or 4 ug of the expression construct for human GAB1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of GAB1 in transfected cells was confirmed by anti-GAB1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, GAB1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24 (4) :1531-1539)
Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42 °C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with GAB1 monoclonal antibody, clone 1AT1979 (Cat # MAB1139) . Data courtesy of Dr. GS Feng at The Burnham Institute.
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq