Recombinant GST fusion protein corresponding to human CDKN1B.
Human, Monkey, Mouse, Rat
Protein A/G affinity chromatography
Flow Cytometry (0.5-1 ug/million cells) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (0.5-1 ug/mL for 30 min at RT) Immunoprecipitation (0.5-1 ug/500 ug protein lysate) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.05% BSA, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq