Urea-denatured recombinant His6 fusion protein corresponding to human CDC20.
Protein A/G affinity chromatography
Flow Cytometry (0.5-1 ug/million cells) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (0.5-1 ug/mL for 30 min at RT) Immunoprecipitation (0.5-1 ug/500 ug protein lysate) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.05% BSA, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq