Flow Cytometry (0.5-1 ug/million cells) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (0.5-1 ug/mL for 30 min at RT) Immunoprecipitation (0.5-1 ug/500 ug protein lysate) Western Blot (0.25-0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.05% BSA, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq