PODXL monoclonal antibody, clone 4F10
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More Files
- More Functions
Immunohistochemistry
Immunohistochemical staining of human angiosarcoma with PODXL monoclonal antibody, clone 4F10 (Cat # MAB11361) at 1:50-1:100 dilution, for 30 min at RT.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PODXL.
Immunogen
A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL.
Host
Mouse
Reactivity
Human, Rabbit, Rat
Form
Liquid
Isotype
IgM
Recommend Usage
Flow Cytometry (5-10 uL/million cells)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100 for 30 min at RT)
Western Blot (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunohistochemical staining of human angiosarcoma with PODXL monoclonal antibody, clone 4F10 (Cat # MAB11361) at 1:50-1:100 dilution, for 30 min at RT.Immunofluorescence
Flow Cytometry
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Gene Info — PODXL
Entrez GeneID
5420Gene Name
PODXL
Gene Alias
Gp200, MGC138240, PC, PCLP
Gene Description
podocalyxin-like
Omim ID
602632Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq
Other Designations
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Interactome
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Disease
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