Mouse monoclonal antibody raised against full length CD47.
Intact CD47 purified from human placenta.
Protein A/G affinity chromatography
Flow Cytometry (0.5-1 ug/million cells) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Frozen sections) (0.5-1 ug/mL for 30 min at RT) Immunoprecipitation (1-2 ug/500 ug protein) Western Blot (0.25-0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.05% BSA, 0.05% sodium azide)
Store at 4°C.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
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