MBP monoclonal antibody, clone 10C12
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: MBP-tagged WLS protein (58.9kDa), Lane 2: MBP-tagged IRF7 protein (59.3kDa).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant MBP (Maltose Binding Protein).
Immunogen
Recombinant protein corresponding to MBP (Maltose Binding Protein) purified from E. coli.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: MBP-tagged WLS protein (58.9kDa), Lane 2: MBP-tagged IRF7 protein (59.3kDa).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.
Zhao C, Denison C, Huibregtse JM, Gygi S, Krug RM.
PNAS 2005 Jul; 102(29):10200.
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Influenza B virus NS1 protein inhibits conjugation of the interferon (IFN)-induced ubiquitin-like ISG15 protein.
Yuan W, Krug RM.
The EMBO journal 2001 Feb; 20(3):362.
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Immunoregulatory properties of ISG15, an interferon-induced cytokine.
D'Cunha J, Knight E Jr, Haas AL, Truitt RL, Borden EC.
PNAS 1996 Jan; 93(1):211.
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Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.
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