Product Browser

Last updated: 2021/4/11

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

Phosphotyrosine monoclonal antibody, clone PY20 

  • Catalog # : MAB11325
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Phosphotyrosine.
  • Immunogen:
  • Phosphotyrosine conjugated to KLH.
  • Host:
  • Mouse
  • Specificity:
  • This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • Flow Cytometry (1-2 ug/million cells)
    Immunofluorescence (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues))
    Immunoprecipitation (1-2 ug/500 ug protein lysate)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human breast with Phosphotyrosine monoclonal antibody, clone PY20 (Cat # MAB11325) at 1:50 using peroxidase-conjugate and DAB chromogen (Note: specific cell surface and cytoplasmic staining).
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • RSS
  • YouTube
  • Linkedin
  • Facebook