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Phosphotyrosine monoclonal antibody, clone PY20

  • Catalog # : MAB11325
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Phosphotyrosine.
  • Immunogen:
  • Phosphotyrosine was used as the immunogen.
  • Host:
  • Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • Western Blot (1:200 -1:2000)
    Immunocytochemistry/Immunofluorescence (1:10 - 1:2000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
    Immunoprecipitation (1:10 - 1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human breast with Phosphotyrosine monoclonal antibody, clone PY20 (Cat # MAB11325) at 1:50 using peroxidase-conjugate and DAB chromogen (Note: specific cell surface and cytoplasmic staining).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
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