Phosphotyrosine monoclonal antibody, clone PY20
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Specification
Product Description
Mouse monoclonal antibody raised against Phosphotyrosine.
Immunogen
Phosphotyrosine conjugated to KLH.
Host
Mouse
Specificity
This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues))
Immunoprecipitation (1-2 ug/500 ug protein lysate)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human breast with Phosphotyrosine monoclonal antibody, clone PY20 (Cat # MAB11325) at 1:50 using peroxidase-conjugate and DAB chromogen (Note: specific cell surface and cytoplasmic staining).Immunofluorescence
Immunoprecipitation
Flow Cytometry
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