Mouse monoclonal antibody raised against Phosphotyrosine.
Phosphotyrosine was used as the immunogen.
Protein G purification
Western Blot (1:200 -1:2000) Immunocytochemistry/Immunofluorescence (1:10 - 1:2000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100) Immunoprecipitation (1:10 - 1:500) The optimal working dilution should be determined by the end user.
In PBS (0.05% BSA, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human breast with Phosphotyrosine monoclonal antibody, clone PY20 (Cat # MAB11325) at 1:50 using peroxidase-conjugate and DAB chromogen (Note: specific cell surface and cytoplasmic staining).