MUC5AC monoclonal antibody, clone 45M1
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Specification
Product Description
Mouse monoclonal antibody raised against MUC5AC.
Immunogen
MUC5AC isolated from the fluid of an ovarian mucinous cyst from an O Le(a-b-) patient.
Host
Mouse
Reactivity
Cat, Chicken, Hedgehog, Human, Monkey, Mouse, Pig, Rabbit, Rat
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1:100 - 1:2000)
Flow Cytometry (1 ug/106 cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human stomach with MUC5AC monoclonal antibody, clone 45M1 (Cat # MAB11324) at 1:500 using peroxidase-conjugate and DAB chromogen.Flow Cytometry
Flow cytometric analysis of fixed (70% ethanol or 80% methanol) and permeabilized A-549 cells using MUC5AC monoclonal antibody, clone 45M1 (Cat # MAB11324) (red) and isotype control (green) at 1 ug/106 cells. -
Gene Info — MUC5AC
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Interactome
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Disease
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Publication Reference
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Comparison of immune response to human rhinovirus C and respiratory syncytial virus in highly differentiated human airway epithelial cells.
Xin-Hui Yuan, Li-Li Pang, Jing Yang, Yu Jin.
Virology Journal 2022 May; 19(1):81.
Application:IF, Human, HBE cell.
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Protostemonine attenuates alternatively activated macrophage and DRA-induced asthmatic inflammation.
Song Y, Wu Y, Li X, Shen Y, Ding Y, Zhu H, Liu F, Yu K, Sun L, Qian F.
Biochemical Pharmacology 2018 Sep; 155:198.
Application:WB-Ti, Mouse, Lung.
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MUC1, MUC2, MUC5AC, and MUC6 in colorectal cancer: expression profiles and clinical significance.
Betge J, Schneider NI, Harbaum L, Pollheimer MJ, Lindtner RA, Kornprat P, Ebert MP, Langner C.
Virchows Archiv 2016 Sep; 469(3):255.
Application:IHC, Human, Colorectal cancer.
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Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance.
Danese E, Ruzzenente O, Ruzzenente A, Iacono C, Bertuzzo F, Gelati M, Conci S, Bendinelli S, Bonizzato G, Guglielmi A, Salvagno GL, Lippi G, Guidi GC.
Surgery 2014 Nov; 156(5):1218.
Application:WB, Human, Bile, Serum.
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Comparison of immune response to human rhinovirus C and respiratory syncytial virus in highly differentiated human airway epithelial cells.
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