Recombinant human Estrogen Receptor alpha protein (amino acidss 2-185).
Protein G purification
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Western Blot (0.5-1.0ug/ml for 2 hours at Room Temp) The optimal working dilution should be determined by the end user.
In 10 mM PBS, pH 7.4 (0.05% BSA and 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human breast invasive ductal carcinoma with ESR1 monoclonal antibody, clone ER505 (Cat # MAB11317) at 1:25 using peroxidase-conjugate and DAB chromogen.
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq