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EPCAM monoclonal antibody, clone MOC-31

  • Catalog # : MAB11315
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against EpCAM (CD326).
  • Immunogen:
  • Neuraminidase treated GLS-1 human small cell lung carcinoma line (de Leij L, 1984). The antibody binding epitope has been mapped to the first EGF-like repeat domain (EGF1) amino acids 27-59 of protein (Balzar, 1999; Balzar 2001). This antibody was assigned to SCLC-Cluster 2 at the Second International Workshop on Lung Cancer Antigens (Souhami, 1984).
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Immunocytochemistry/Immunofluorescence (1:10-1:2000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Bioreactor concentrate (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human colon cancer with EPCAM monoclonal antibody, clone MOC-31 (Cat # MAB11315) at 1:200 using peroxidase-conjugate and DAB chromogen (Note: specific membrane staining).
  • Immunocytochemistry
  • Immunofluorescence
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 4072
  • Gene Name:
  • Gene Alias:
  • 17-1A,323/A3,CD326,CO-17A,CO17-1A,EGP,EGP-2,EGP34,EGP40,ESA,Ep-CAM,GA733-2,HEA125,KS1/4,KSA,M4S1,MH99,MIC18,MK-1,MOC31,TACST-1,TACSTD1,TROP1,hEGP-2
  • Gene Description:
  • epithelial cell adhesion molecule
  • Gene Summary:
  • This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
  • Other Designations:
  • adenocarcinoma-associated antigen,carcinoma-associated antigen GA733-2,human epithelial glycoprotein-2,membrane component, chromosome 4, surface marker (35kD glycoprotein),tumor-associated calcium signal transducer 1
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