Mouse monoclonal antibody raised against EpCAM (CD326).
Neuraminidase treated GLS-1 human small cell lung carcinoma line (de Leij L, 1984). The antibody binding epitope has been mapped to the first EGF-like repeat domain (EGF1) amino acids 27-59 of protein (Balzar, 1999; Balzar 2001). This antibody was assigned to SCLC-Cluster 2 at the Second International Workshop on Lung Cancer Antigens (Souhami, 1984).
Immunocytochemistry/Immunofluorescence (1:10-1:2000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:400) The optimal working dilution should be determined by the end user.
Bioreactor concentrate (0.05% sodium azide)
Store at 4°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human colon cancer with EPCAM monoclonal antibody, clone MOC-31 (Cat # MAB11315) at 1:200 using peroxidase-conjugate and DAB chromogen (Note: specific membrane staining).
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq