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Pan Cytokeratin monoclonal antibody, clone AE1 + AE3 

  • Catalog # : MAB11311
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Pan Cytokeratin.
  • Immunogen:
  • Human epidermal keratin.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 40-67
  • Reactivity:
  • Human
  • Specificity:
  • This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8); 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19). AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Flow Cytometry (1-2 ug/million cells)
    Immunofluorescence (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
    Western Blot (1-2 ug/mL for 2 hours at RT)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon carcinoma with Pan Cytokeratin monoclonal antibody, clone AE1 + AE3 (Cat # MAB11311).
  • Immunofluorescence
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cytometric analysis of human Pan-Cytokeratins on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Pan Cytokeratin monoclonal antibody, clone AE1 + AE3.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Gene Name:
  • KRT77
  • Gene Alias:
  • K1B,KRT1B,MGC148087
  • Gene Description:
  • keratin 77
  • Gene Summary:
  • Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13
  • Other Designations:
  • keratin 1B
  • Gene Information
  • Gene Name:
  • KRT76
  • Gene Alias:
  • HUMCYT2A,KRT2B,KRT2P
  • Gene Description:
  • keratin 76
  • Gene Summary:
  • Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq
  • Other Designations:
  • cytokeratin 2,keratin 2p
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