Western Blot (1-3 ug/ml) Immunocytochemistry/Immunofluorescence (1:10 - 1:2000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:400) Immunoprecipitation (1:10 - 1:500) The optimal working dilution should be determined by the end user.
In PBS (0.05% BSA, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon cancer with KRT18 monoclonal antibody, clone DA7 (Cat # MAB11308) at 1:200 using peroxidase-conjugate and DAB chromogen (Note: cytoplasmic staining of tumor cells).
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
cell proliferation-inducing protein 46,cytokeratin 18