A membrane preparation of a small cell lung carcinoma.
This antibody recognizes two proteins (185 kDa & 145 kDa), identified as two isoforms of neural cell adhesion molecule (NCAM1/CD56).
Protein G purification
Western Blot (1:100-1:2000) Flow Cytometry (1-2 ug/106 cells) Immunocytochemistry/Immunofluorescence (1:10-1:2000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:200) Immunoprecipitation (1:10-1:500) The optimal working dilution should be determined by the end user.
In PBS (0.05% BSA, 0.05% sodium azide)
Store at 4°C.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon ganglion cells with NCAM1 monoclonal antibody, clone 123C3.D5 (Cat # MAB11301) at 1:100 using peroxidase-conjugate and DAB chromogen. Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0.
Flow cytometric analysis of human monocytes using NCAM1 monoclonal antibody, clone 123C3.D5 (Cat # MAB11301) at 1 ug/106 cells.