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NCAM1 monoclonal antibody, clone 123C3

  • Catalog # : MAB11301
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against NCAM1.
  • Immunogen:
  • A membrane preparation of a small cell lung carcinoma.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This antibody recognizes two proteins (185 kDa & 145 kDa), identified as two isoforms of neural cell adhesion molecule (NCAM1/CD56).
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Western Blot (1:100-1:2000)
    Flow Cytometry (1-2 ug/106 cells)
    Immunocytochemistry/Immunofluorescence (1:10-1:2000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:200)
    Immunoprecipitation (1:10-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human colon ganglion cells with NCAM1 monoclonal antibody, clone 123C3 (Cat # MAB11301) at 1:100 using peroxidase-conjugate and DAB chromogen. Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of human monocytes using NCAM1 monoclonal antibody, clone 123C3 (Cat # MAB11301) at 1 ug/106 cells.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 4684
  • Gene Name:
  • NCAM1
  • Gene Alias:
  • CD56,MSK39,NCAM
  • Gene Description:
  • neural cell adhesion molecule 1
  • Other Designations:
  • antigen recognized by monoclonal antibody 5.1H11,neural cell adhesion molecule, NCAM
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