Mouse monoclonal antibody raised against CD45RO (PTPRC).
Interleukin-2-dependent human T lymphocytes.
Protein G purification
Western Blot (2-5 ug/ml) Flow Cytometry (0.5 - 2 ug/ml) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:250 - 1:500) The optimal working dilution should be determined by the end user.
In PBS (0.05% BSA, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human tonsil with CD45RO monoclonal antibody, clone UCHL-1 (Cat # MAB11300) at 1:500 using peroxidase-conjugate and DAB chromogen (Note: membrane staining).
Flow cytometric analysis of lymphocyte gated population of PBMC using either CD45RO monoclonal antibody, clone UCHL-1 (Cat # MAB11300) (0.5ug/106 cells) or isotype control following surface staining protocol. PE-conjugated goat anti-mouse IgG secondary antibody is used to identify the positive staining on PPI negative lymphocyte gated population.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
CD45 antigen,T200 glycoprotein,T200 leukocyte common antigen,glycoprotein,leukocyte-common antigen,protein tyrosine phosphatase, receptor type, c polypeptide