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IL33 monoclonal antibody, clone 4E9 

  • Catalog # : MAB1130
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human IL33.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 112-270 of human IL33.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: 293T cell lysate, Lane 2: Jurkat cell lysate.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of Jurkat cells. The cell was stained with IL33 monoclonal antibody, clone 4E9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of Jurkat cells. The cell was stained with IL33 monoclonal antibody, clone 4E9 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • IL33
  • Gene Alias:
  • C9orf26,DKFZp586H0523,DVS27,NF-HEV,NFEHEV,RP11-575C20.2
  • Gene Description:
  • interleukin 33
  • Other Designations:
  • DVS27-related protein,OTTHUMP00000021041,nuclear factor from high endothelial venules
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